Daytona hits $1B valuation with $60M Series B for AI agent cloud

Daytona has landed a $60 million Series B and a $1 billion valuation, pushing its total funding to $91 million. FirstMark led the round, with backing from a long list that includes Patrick Collison, Adam D’Angelo, Brendan Foody, Tobi Lütke, Olivier Pomel, Aravind Srinivas and Eric Yuan.

That investor mix tells you plenty. This is not just another infrastructure raise; it is a bet that AI agents are moving from demos to real work, and that real work needs containment. Daytona’s pitch is simple enough: give each agent a secure, isolated computing environment, so it can run code, use tools and complete tasks without wandering into someone else’s mess.

The company says its platform lets developers and enterprises train, evaluate, deploy and manage agents through a single API. It supports Linux, Windows and macOS, and the compute can scale up or down depending on the task. Agents can also pause, resume and try another approach without losing progress, which is exactly the kind of boring plumbing that becomes valuable once the shiny part wears off.

Daytona says it already provides infrastructure to Anthropic, OpenAI, NVIDIA, Cursor and Cognition. Its customer list also includes Meta, Cohere, Mercor, Handshake, Surge AI, SambaNova, Postman, Bolt.new, n8n and Clay. The company reports a 25-fold jump in revenue run rate between January and September, and says Snorkel and Trajectory are using its infrastructure to cut processing times.

The new money will go into expanding beyond the initial sandbox product, scaling compute and hiring across San Francisco, Croatia and Singapore. Pace Capital doubled its commitment, Upfront Ventures tripled theirs and E2VC quadrupled theirs. In a market where everyone wants agents to do more, Daytona is selling the less glamorous promise: if the agent breaks something, it should only break its own little box.